Antigen

Antigen Class

TFs and others

Antigen

Mafg

Cell type

Cell type Class

Neural

Cell type

Astrocytes

MeSH Description

A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Sample

source_name

EAE_IP_astrocytes

strain

C57BL/6

genotype/variation

wild type

Sex

female

sample group

experimental autoimmune encephalomyelitis (EAE)

cell type

Flow cytometry sorted astrocytes

chip antibody

MAFG antibody (Genetex, GTX114541)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Each CNS was isolated per mouse. A cell suspension was generated by trypsinization, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, washed in PBS, and stained with primary antibodies for flow cytometry. Once cells were sorted, chromatin was isolated using the Active Motif ChIP-IT Express Enzymatic shearing kit. Chromatin complexes were IPed with MAFG antibody (Genetex, GTX114541), cross-links were reversed, and DNA was cleaned up for library prep using QIAquick PCR purification kit. NEBNext Ultra II DNA Library Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

74478635

Reads aligned (%)

92.8

Duplicates removed (%)

86.0

Number of peaks

190 (qval < 1E-05)

mm9

Number of total reads

74478635

Reads aligned (%)

92.7

Duplicates removed (%)

86.1

Number of peaks

158 (qval < 1E-05)